If you do not see that datastore is ticked then it is mean that that datastore is not enabled for datastore-heart-beating for sure.
In your case, folder is still visible, it is mean that that datastore was selected as datastore-heartbeat earlier but now it is disabled. So you need not to worry.
Also, as I said, VI client connected to vCenter server will show you whether datastore is enabled for heartbeating. Just right click on datastore>>unmount>> window will show you all the checks (red : means enabled for heartbeating) : When you click on "unmount" it will not immediately unmount the datastore until you click on OK button. So you need to worry if you want to verify by this way as well.